Assuming you have just acquired a pristine, scanned, or reprinted manual, follow this checklist:
Never exceed the maximum safe heater current. Overheating deforms the platinum coil, permanently altering your pull profiles.
The is a cornerstone in laboratory pipette fabrication, renowned for its ability to produce consistent, high-quality micropipettes for applications like patch clamping and microinjection . Although Narishige has transitioned to the PC-100 , the PC-10 remains a workhorse in many labs worldwide. narishige pc10 manual new
The Narishige PC-10 is a classic, dual-stage vertical micropipette puller highly regarded in electrophysiology, microinjection, and cellular biology laboratories worldwide. It utilizes gravity and a multi-stage heating element to draw glass capillaries into consistent, high-quality micropipettes or microinjection needles.
| Heat setting | Weight | Resulting pipette resistance (3 mm tip) | |--------------|--------|------------------------------------------| | 55 | 150g | No separation – too cold | | 62 | 150g | 2–3 MΩ (good for whole-cell patch) | | 68 | 150g | 5–7 MΩ (sharp intracellular) | | 75 | 200g | 10–12 MΩ (too long taper, fragile) | | 80+ | 100g | Blunt pipette – overheated glass | Assuming you have just acquired a pristine, scanned,
Your (e.g., patch-clamp, microinjection, or extracellular recording)
Insert the leads of the Narishige heating coil into the terminals. Centering the coil perfectly around the vertical path of the glass capillary is critical. Although Narishige has transitioned to the PC-100 ,
The Ultimate Guide to the Narishige PC-10 Glass Microelectrode Puller: Operation, Calibration, and Maintenance
The Narishige PC-10 manual is a comprehensive guide that provides users with detailed information on the instrument's operation, maintenance, and troubleshooting. The manual covers essential topics such as:
The PC-10 features an intuitive, analogue-digital hybrid interface designed for quick adjustment. Turns the unit on or off. Mode Selector (1-Stage / 2-Stage):
In this configuration, the puller applies a single continuous heating phase. The weight blocks pull the glass capillary downwards in one smooth motion.